Ofo will no longer be operating in Kings Hedges, Arbury and Chesterton areas of Cambridge.
The dockless bike-sharing company made the announcement to its users ahead of the winter period – opting instead for a higher density of bikes for the centre of Cambridge.
The company said those areas are "not visited" by the bike users which made it "complicated" for its team to manage.
The dockless bikes can be unlocked through an app, where you pay with your payment card details.
The company shrunk the area where you could unlock and park the bikes in August, and also introduced an automatic £10 charge if you took the bike out of that area.
But since then ofo has decided to shrink the area even further.
The areas available to ofo bike users are highlighted in the map on the city on the Ofo app with a green geofence.
The message sent to its users said: "With the winter period approaching we have reviewed our operation.
"As such, today we are announcing an important change concerning our operating area.
"Some areas are not visited by our users and are unfortunately more complicated to manage by our teams.
"That's why we made the decision to reduce some areas of winter.
"This modification will provide a higher density of bikes and better service for the majority of our users."
What has happened with ofo?
Ofo has 450 bikes on the city's streets but the company has come under fire as many get abandoned and some are even handed in to the police station when locks are broken or they have been found abandoned by the public.
From one being hung up in the Princess Diana Memorial Gardens, to another going for a dip near Abbey swimming pool, the bikes are ending up in some inappropriate places.
Several ofo bikes were found dumped at the bottom of the River Cam and the Cherry Hinton Brook in August.
Video footage captured in East Chesterton has even shown a pair of vandals hitting the ofo bikes with rocks.
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Three youngsters are seen in a video standing by the ofo bike in Fallowfields before the younger one runs off as a van drives past.
The two older youths then start to throw the bike about and use a large rock in an attempt to smash it up – while appearing to be eating ice cream.
Matthew Thomas-Keeping, ofo's operations manager for Cambridge, said the company eventually wanted to have around 3,000 bikes in the city.